HPLC 测定不同基原、不同部位吴茱萸中 4 种生物碱的含量及其质量评价

摘要：

【目的】建立高效液相色谱（HPLC）法同时测定吴茱萸中4种生物碱含量的方法，评价不同基原、不同药用部位吴茱萸的质量。【方法】采用HPLC法测定吴茱萸、石虎和疏毛吴茱萸的果皮和种子中去氢吴茱萸碱、吴茱萸新碱、吴茱萸碱及吴茱萸次碱4种生物碱的含量。【结果】果皮中去氢吴茱萸碱、吴茱萸碱和吴茱萸次碱的含量均高于种子；种子中吴茱萸新碱的含量 高于果皮。在果皮中，吴茱萸碱与吴茱萸次碱的含量高于疏毛吴茱萸；在种子中，吴茱萸碱与吴茱萸次碱的含量低于疏毛吴茱萸。【结论】去氢吴茱萸碱、吴茱萸碱和吴茱萸次碱主要分布在吴茱萸果皮中，吴茱萸新碱主要分布于吴茱萸种子中；吴茱萸果皮中吴茱萸碱与吴茱萸次碱的含量高于疏毛吴茱萸果皮，吴茱萸种子中吴茱萸碱与吴茱萸次碱的含量低于疏毛吴茱萸种子。本研究为吴茱萸药材质量标准的制定及吴茱萸药材差异化利用策略提供了依据。

Abstract:

Objective] A high performance liquid chromatography (HPLC) method is established for the simultaneous determination of four alkaloids in Evodia rutaecarpa, and the quality of Evodia rutaecarpa from different origins and different medicinal parts is also evaluated. [Methods] The contents of four alkaloids, namely dehydroevodiamine, evodiamine, rutaecarpine and rutaecarpamine, are determined by HPLC. [Results] In the same origin with different medicinal parts: the contents of dehydroevodiamine, evodiamine and rutaecarpine in pericarps are higher than those in seeds. The content of evocarpine in seeds is higher than that in pericarps. In the same medicinal part with different origins: In pericarps, the content of evodiamine and rutaecarpine in E. rutaecarpa (Juss.) Benth. are higher than those in E. rutaecarpa (Juss.) Benth. var. officinalis (Dode) Huang, while in seeds, the contents of the compounds mentioned above in E. rutaecarpa (Juss.) Benth are even lower. [Conclusion] Dehydrorutaine, evodiamine and rutaecarpine are mainly distributed in the pericarps of Fructus Euodiae, while evodiaine is mainly distributed in seeds. The contents of evodiamine and rutaecarpine in the pericarp of E. rutaecarpa (Juss.) Benth. are higher than those in the pericarp of E. rutaecarpa (Juss.) Benth. var. bodinieri (Dode) Huang; while the contents of evodiamine and rutaecarpine in the seeds of E. rutaecarpa (Juss.) Benth. are lower than those in the seeds of E. rutaecarpa (Juss.) Benth. var. bodinieri (Dode) Huang.This study provides a preliminary research basis for the quality control and rational application of different medical parts of Fructus Euodiae. 

引用格式：陈萌，刘斯琪，杜玥瑾，等.HPLC 测定不同基原、不同部位吴茱萸中 4 种生物碱的含量及其质量评价[J].标准科学，2025(10):99-106.

作者简介：陈萌，博士，助理研究员，研究方向为健康服务与中医药标准化。 刘斯琪，博士，助理研究员，研究方向为中药药效物质与质量标准。 杜玥瑾，硕士，主治医师，研究方向为健康服务。 侯非，硕士，研究员，研究方向为健康服务标准化。 王娜娜，硕士，副研究员，研究方向为健康服务标准化。 郑娟尔，博士，研究员，研究方向为健康服务标准化。 付强，通信作者，博士，研究员，研究方向为健康服务与中医药标准化。